Construct · 1 fields · 1 citations

transfection: a standalone construct

One name. One construct. A landmark on the map.

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transfection1 CONSTRUCTTransfectionGRAHAM & VAN DER EB · 1973

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Molecular biology

Introducing nucleic acid into eukaryotic cells

In molecular biology, transfection is the deliberate introduction of foreign nucleic acid into eukaryotic cells, so that the delivered DNA or RNA is taken up and, in stable transfection, maintained and expressed. Graham and van der Eb's 1973 A new technique for the assay of infectivity of human adenovirus 5 DNA established the calcium-phosphate method that made routine mammalian-cell transfection possible. The referent is a gene-delivery event into cultured cells, measured by uptake and by expression of the introduced sequence, and is the eukaryotic counterpart to bacterial transformation.[1]

Unit
The fraction of cells that take up and express the introduced nucleic acid
Recovery means
Not applicable
Measured by
Quantified by transfection efficiency, such as the proportion of cells expressing a reporter or the number of stable integrants recovered

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Nearby constructs

assay1 fields · 1 citationsanchor · a standalone constructevent3 fields · 5 citationsjingle · one word, three constructsexpression4 fields · 4 citationsjingle · one word, four constructs

References

  1. Graham, F. L., & van der Eb, A. J. (1973). A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology, 52(2), 456–467. https://doi.org/10.1016/0042-6822(73)90341-3

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