Construct · 1 fields · 1 citations
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In molecular biology the comet assay (single-cell gel electrophoresis) is a technique for measuring DNA damage in individual cells: cells are embedded in gel, lysed, and subjected to an electric field, so that broken or relaxed DNA fragments migrate out of the nucleoid and form a bright streaming "tail" while intact DNA stays in the "head," the whole figure resembling a comet. Singh, McCoy, Tice, and Schneider's 1988 A simple technique for quantitation of low levels of DNA damage in individual cells introduced the sensitive alkaline version now in standard use. Here "comet" names a visual scoring pattern under the microscope, quantified by tail length and the fraction of DNA in the tail: a laboratory readout, not a body in space or a pyrotechnic effect.[1]
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