Construct · 1 fields · 1 citations

library: a standalone construct

One name. One construct. A landmark on the map.

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library1 CONSTRUCTDNA libraryMANIATIS · CLONED DNA

A single standalone construct; nearby constructs are located against it in the Collider. Tap a field for its definition, unit, and measures.

Molecular biology and genomics

A collection of cloned DNA fragments representing a source's sequences

A collection of cloned DNA fragments, held in host cells or vectors, that together represent the sequences present in a source genome or set of transcripts. Maniatis and colleagues' 1978 The isolation of structural genes from libraries of eucaryotic DNA described building such collections and screening them to recover individual genes, establishing the genomic library as a working laboratory tool. The referent is a biological archive of DNA inserts that can be searched for a sequence of interest, sized by clone number and coverage, and it is unrelated to an institution of books or a package of software routines.[1]

Unit
A single collection of cloned DNA fragments held in host cells or vectors
Recovery means
Not applicable
Measured by
Assessed by number of clones, insert size, and genome or transcriptome coverage

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Nearby constructs

archive1 fields · 1 citationsanchor · a standalone constructindex5 fields · 11 citationsjingle · one word, five constructsretrieval3 fields · 3 citationsjingle · one word, three constructs

References

  1. Maniatis, T., Hardison, R. C., Lacy, E., Lauer, J., O'Connell, C., Quon, D., Sim, G. K., & Efstratiadis, A. (1978). The isolation of structural genes from libraries of eucaryotic DNA. Cell, 15(2), 687–701. https://doi.org/10.1016/0092-8674(78)90036-3

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