Construct · 1 fields · 1 citations

Gibson assembly: a standalone construct

One name. One construct. A landmark on the map.

● anchor · a standalone construct Nearest construct by meaning·not indexed

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Synthetic biology

joining overlapping DNA fragments in one isothermal reaction using exonuclease, polymerase and ligase together.

In synthetic biology, Gibson assembly refers to joining overlapping DNA fragments in one isothermal reaction using exonuclease, polymerase and ligase together. This meaning comes from Gibson (2009) in Nature Methods.[1]

Unit
Recovery means
Measured by

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Nearby constructs

assembly1 fields · 1 citationsanchor · a standalone construct polymerase1 fields · 1 citationsanchor · a single construct reaction4 fields · 4 citationsjingle · one word, four constructs

References

Gibson, D. G., Young, L., Chuang, R., Venter, J. C., Hutchison, C. A., & Smith, H. O. (2009). Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods, 6(5), 343-345. https://doi.org/10.1038/nmeth.1318

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