Construct · 1 fields · 1 citations

shuttle: a standalone construct

One name. One construct. A landmark on the map.

● anchor · a standalone construct Nearest construct by meaning·not indexed
shuttle1 CONSTRUCTShuttle vectorSTRUHL · 1979

A single standalone construct; nearby constructs are located against it in the Collider. Tap a field for its definition, unit, and measures.

Molecular biology

an engineered DNA vector that replicates in two host species, such as yeast and Escherichia coli.

In molecular biology a shuttle vector is a piece of DNA engineered to replicate in two different host species (classically yeast and the bacterium Escherichia coli) so a gene can be amplified in one and studied in the other. Struhl and colleagues' 1979 High-frequency transformation of yeast: Autonomous replication of hybrid DNA molecules built the hybrid plasmids that made this shuttling routine. The referent is a laboratory DNA construct that carries genes back and forth between organisms, named for the weaver's tool but otherwise unrelated to cloth or sport.[1]

Unit
A DNA construct carrying genes between two host species
Recovery means
Not applicable
Measured by
By transformation efficiency and stable autonomous replication in each host

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Nearby constructs

carrier3 fields · 3 citationsjingle · one word, three constructs

References

  1. Struhl, K., Stinchcomb, D. T., Scherer, S., & Davis, R. W. (1979). High-frequency transformation of yeast: Autonomous replication of hybrid DNA molecules. Proceedings of the National Academy of Sciences, 76(3), 1035–1039. https://doi.org/10.1073/pnas.76.3.1035

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