Construct · 2 fields · 4 citations

Where does blot collide?

One name. Different field. Different construct. One map.

● jingle · one word, two constructs Nearest construct by meaning·not indexed
blot2 CONSTRUCTSMembrane blotSOUTHERN · DNA TRANSFERInkblot testRORSCHACH · INKBLOT

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1975Membrane blot2003Inkblot test
2026

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Molecular biology

Molecules transferred to a membrane and probed

In molecular biology a blot is the product of transferring size-separated molecules out of a gel onto a membrane, where they are immobilised and then detected with a labelled probe. Southern's 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis established the technique for DNA, and the method was extended to RNA (the Northern blot of Alwine, Kemp, and Stark, 1977) and to proteins (the electrophoretic protein transfer of Towbin, Staehelin, and Gordon, 1979, later called the Western blot). The referent is a detection assay (a pattern of bands on a membrane read to confirm the presence and size of a target molecule), and it has nothing in common with a projective inkblot or a vulnerable backgammon checker beyond the word.[1][2][3]

Unit
The banding pattern of target molecules captured on a membrane
Recovery means
Not applicable
Measured by
Read as the position and intensity of probe-detected bands, calibrated against molecular-weight or size markers

Projective psychology

An ambiguous inkblot read for personality

In projective psychology a blot is an ambiguous inkblot shown to a respondent, whose descriptions of what they see are scored as a window onto perception and personality. The method originates with Hermann Rorschach's symmetrical inkblots, and Exner's The Rorschach: A comprehensive system (2003) standardised how responses are coded by location, determinants, and content. The referent is a projective stimulus and its scored response, interpreted as a psychological measure, and it bears no relation to a molecular transfer assay or a backgammon checker beyond the common word 'blot'.[4]

Unit
The coded responses a person gives to an ambiguous inkblot
Recovery means
Not applicable
Measured by
Scored by standardized systems coding response location, determinants, form quality, and content

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Nearby collisions

probe3 fields · 3 citationsjingle · one word, three constructsassay1 fields · 1 citationsanchor · a standalone construct

References

  1. Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98(3), 503–517. https://doi.org/10.1016/S0022-2836(75)80083-0
  2. Alwine, J. C., Kemp, D. J., & Stark, G. R. (1977). Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of Sciences, 74(12), 5350–5354. https://doi.org/10.1073/pnas.74.12.5350
  3. Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences, 76(9), 4350–4354. https://doi.org/10.1073/pnas.76.9.4350
  4. Exner, J. E. (2003). The Rorschach: A comprehensive system, Vol. 1: Basic foundations and principles of interpretation (4th ed.). John Wiley & Sons. ISBN 9780471386728

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