Construct · 1 fields · 2 citations

primer: a standalone construct

One name. One construct. A landmark on the map.

● anchor · a standalone construct Nearest construct by meaningprimingcompare the meanings
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primer1 CONSTRUCTDNA primerKLEPPE · PRIMED REPAIR

A single standalone construct; nearby constructs are located against it in the Collider. Tap a field for its definition, unit, and measures.

Molecular biology

Short strand that starts DNA synthesis

Kleppe, Ohtsuka, Kleppe, Molineux, and Khorana's Studies on polynucleotides (1971) set out repair replication, in which a short synthetic strand bound to a template gives a DNA polymerase the free 3' end it needs to begin copying, the founding description of a primer's role. Mullis and Faloona's Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction (1987) made the primer central to the polymerase chain reaction, where two primers flank a target and are extended in repeated cycles to amplify it. Here a primer is a short nucleic-acid strand, a few dozen bases long, that initiates copying of a genetic template, and it bears no relation to a coat of paint or a children's schoolbook.[1][2]

Unit
One oligonucleotide primer, a strand of a few dozen bases
Recovery means
Not applicable
Measured by
Specified by base sequence, length, and melting temperature, and checked by gel or capillary assays of the product

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Nearby constructs

adhesion4 fields · 4 citationsjingle · one word, four constructsannealing3 fields · 5 citationsjingle · one word, three constructsamplification4 fields · 4 citationsjingle · one word, four constructs

References

  1. Kleppe, K., Ohtsuka, E., Kleppe, R., Molineux, I., & Khorana, H. G. (1971). Studies on polynucleotides: XCVI. Repair replication of short synthetic DNAs as catalyzed by DNA polymerases. Journal of Molecular Biology, 56(2), 341-361. https://doi.org/10.1016/0022-2836(71)90469-4
  2. Mullis, K. B., & Faloona, F. A. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology, 155, 335-350. https://doi.org/10.1016/0076-6879(87)55023-6

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